Moss Laboratory Protocols


Important Links

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Culturing and harvesting techniques

Amoeba Culture

Maciver Lab Protocols

"M" medium protocol (old photocopy)

Chlamydomonas media (from

Chlamydomonas Methods

Environmental Analysis

Phosphate Analysis

Chlorophyll a Analysis

DNA Protocols

               Chloroform Isoamyl Alcohol Phenol: CIP Methodology  

  CTAB / Hot Detergent

Specific PCR Protocols

Protein Protocols

Protein Harvesting Considerations

Gel Electrophoresis Methods



DNA Agarose Gels


Microscopy Protocols

Electron Microscopy:

LKB Knifebreaker

Trimming and sectioning

Infiltration and embedment: Spurrs

Infiltration and embedment: Durcupan ACM



"Simultaneous" fixation protocol for gelatinous organisms

Muscle fixation

Coral fixation methods

Fixation for subsequent immunostaining



Staining of sections: TEM and LM

Toluidine Blue O for light microscopy of 1/4 micron sections

Uranyl Acetate / Silver Nitrate / Lead Citrate methods

Reduction of Autofluorescence (From WCIF)

Electrophysiology Techniques

Coming soon...


Laboratory Materials Handling

is regulated by

Auburn University Risk Management and Safety

Risk Management Homepage

316 Leach Science Center
Auburn University, AL 36849

Fax: 334-844-4640
Hours: 7:45 - 4:45

Chemicals are inventoried at a central server location under the...

Chematix Program

Check that website for up-to-date information on training at chemicals handling



Shawn McNulty, Laboratory Safety Program Manager/ Biosafety Officer   Cell phone: 740-9711  

Michael Freeman, Hazardous Materials Technician  Cell phone: 740-1267

Mike Anderson, Radiological Safety Officer  Cell phone: 750-9010

Sevgi Kucuktas, Radiation Safety Specialist  Cell phone: 703-0164, Office 844-6238



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