Moss Laboratory Protocols
and
Important Links
yellow: not filled in
Culturing and harvesting techniques
"M" medium protocol (old photocopy)
Chlamydomonas media (from www.chlamy.org)
Environmental Analysis
DNA Protocols
Chloroform Isoamyl Alcohol Phenol: CIP Methodology
Specific PCR Protocols
Protein Protocols
Protein Harvesting Considerations
Gel Electrophoresis Methods
DGGE
Microscopy Protocols
Electron Microscopy:
Trimming and sectioning
Infiltration and embedment: Spurrs
Infiltration and embedment: Durcupan ACM
Fixation
"Simultaneous" fixation protocol for gelatinous organisms
Muscle fixation
Coral fixation methods
Fixation for subsequent immunostaining
Mordanting
Staining of sections: TEM and LM
Toluidine Blue O for light microscopy of 1/4 micron sections
Uranyl Acetate / Silver Nitrate / Lead Citrate methods
Reduction of Autofluorescence (From WCIF)
Electrophysiology Techniques
Coming soon...
Laboratory Materials Handling
is regulated by
Auburn University Risk Management and Safety
316 Leach Science Center
Auburn University, AL 36849
334-844-4870
Fax: 334-844-4640
Hours: 7:45 - 4:45
Chemicals are inventoried at a central server location under the...
Check that website for up-to-date information on training at chemicals handling
Contacts:
Shawn McNulty, Laboratory Safety Program Manager/ Biosafety Officer Cell phone: 740-9711Michael Freeman, Hazardous Materials Technician Cell phone: 740-1267
Mike Anderson, Radiological Safety Officer Cell phone: 750-9010Sevgi Kucuktas, Radiation Safety Specialist Cell phone: 703-0164, Office 844-6238
