Goodwin Laboratory

Department of Chemistry and Biochemistry

Auburn University 

Follow us on Twitter:  @AuburnRadical

Research Interests                                                                                   

The common threads that join our research interests together are the topics of bacterial virulence, antibiotic resistance, and discovery of new leads for antibiotic development. Toward the latter, we are intersted in new leads that will combat infection by Mycobacterium tuberculosis. We are also interested in compounds to address a variety of fungal and oomycete agricultural pathogens. We focus on three main systems. Read more about each one by following the corresponding links.

1) the multifunctional heme enzyme known as KatG,
2) two enzymes essential for aromatic amino acid biosynthesis, shikimate kinase and shikimate dehydrogenase, and
3) antimicrobial compounds generated by Bacillus and Paenibacillus biosynthetic gene clusters

Group Photos
      November 2023


Current Lab Members

Marks Family Scholarship

Lab Alumni

Goodwin Lab Pics


Apply to our Graduate Program

KatG Structure and Radicals


Basak, S., Alam, J., Goodwin, D., Harris, J., Patel, J.D., McCullough, P., and McElroy, J.S. "Detecting ACCase-targeting herbicides effect on ACCase activity utilizing a malachite green colorimetric functional assay" Weed Sci. 2022, 70, 14 - 19.

de Faria, C.F., Moreira, T., Lopes, P., Costa, H., Krewall, J.R., Barton, C.M., Santos, S., Goodwin D.C., Mochado, D., Viveiros, M., Machuqueiro, M., and Martins, F. "Designing new antitubercular isoniazid derivatives with improved reactivity and membrane trafficking abilities. Biomed. Pharmacother. 2021, 144, 112362.

Krewall, J.R.; Minton, L.E.; Goodwin, D.C. "KatG structure and mechanism: Using protein-based oxidation to confront the threats of reactive oxygen" In Bridging Structure and Function in Mechanistic Enzymology. J.M. Miller, Ed., 2020, American Chemical Society, Washington, DC, pp. 83-120.

Sahrmann, P.G.; Donnan, P.H.; Merz, K.M.; Mansoorabadi, S.O.; Goodwin, D.C. " A parameterizer of post-translationally modified residues" J. Chem. Inf. Model. 2020, 60, 4424.

Simithy, J.; Fuanta, N.R.; Alturki, M.; Hobrath, J.V.; Wahba, A. E.; Pina, I.; Rath, J.; Hamann, M.T.; DeRuiter, J.; Goodwin, D.C.; Calderon, A.I."Slow-binding inhibition of Mycobacterium tuberculosis shikimate kinase by manzamine alkaloids" Biochemistry 2018, 57, 4923.

Simithy, J.; Fuanta, N.R.; Kochanowska-Karamayan, A.; Hobrath, J.V.; Hamann, M.T.; Goodwin, D.C.; Calderon, A.I. "Mechanism of irreversible inhibition of Mycobacterium tuberculosis shikimate kinase by ilimaquinone" Biochim. Biophys. Acta 2018, 1866, 731.

Alturki, M.S.; Fuanta, N.R.; Jarrard, M.A.; Hobrath, J.V.; Goodwin, D.C.; Rants'o, T.A.; Calderon, A.I. "A multifaceted approach to identify non-specific enzyme inhibition: Application to Mycobacterium tuberculosis shikimate kinase" Bioorg. Medicin. Chem. Lett. 2018, 28, 802.

Njuma, O.J.; Davis, I.; Ndontsa, E.N.; Krewall, J.R.; Liu, A.; Goodwin, D.C. "Mutual synergy between catalase and peroxidase activities of the bifunctional enzyme KatG is facilitated by electron-hole hopping within the enzyme" J. Biol. Chem. 2017, 292, 18408

McCarty, S.E.; Schellenberger, A.; Goodwin, D.C.; Fuanta, N.R.; Tekwani, B.L.; Calderon, A.I. "Plasmodium falciparum thioredoxin reductase (PfTrxR) and its role as a target for new antimalarial discovery" Molecules 2015, 20, 11459.

Gordon, S.; Simithy, J.; Goodwin, D.C.; Calderon, A.I. "Selective Mycobacterium tuberculosis shikimate kinase inhibitors as potential antibacterials" Perspect. Medicin. Chem. 2015, 7, 9.

Huang, J.; Smith, F., Panizzi, J.R.; Goodwin, D.C.; Panizzi, P. "Inactivation of myeloperoxidase by benzoic acid hydrazide" Arch. Biochem. Biophys. 2015, 570, 14.

Kudalkar, S.N.; Njuma, O.J.; Li, Y.; Muldowney, M.; Fuanta, N.R.; Goodwin, D.C. "A role for catalase-peroxidase large loop 2 revealed by deletion mutagenesis: Control of active site water and ferric enzyme reactivity" Biochemistry 2015, 54, 1648.

Simithy, J.; Gill, G.; Wang, Y.; Goodwin, D.C.; Calderon, A.I. "Development of an ESI-LC-MS based assay for kinetic evaluation of M. tuberculosis shikimate kinase" Anal. Chem. 2015, 87, 2129.

Njuma, O.J.; Ndontsa, E.N.; Goodwin, D.C.  "Catalase in peroxidase clothing: Interdependent cooperation of two cofactors in the catalytic versatility of KatG" Arch. Biochem. Biophys. 2014, 544, 27.

Wang, Y.; Goodwin D.C.  "Integral role of the I'-helix in the function of the "inactive" C-terminal domain of catalase-peroxidase (KatG)" Biochim. Biophys. Acta 2013, 1834, 362.

Kudalkar, S.N.; Campbell, R.A.; Li, Y.; Varnado, C.L.; Prescott, C.; Goodwin, D.C.  "Enhancing the peroxidatic activity of KatG by deletion mutagenesis" J. Inorg. Biochem. 2012, 116, 106.

Ndontsa, E.N.; Moore, R.L.; Goodwin, D.C.  "Stimulation of KatG catalase activity by peroxidatic electron donors"  Arch. Biochem. Biophys. 2012, 105, 215.

Tejero, J.; Biswas, A.; Haque, M.M.; Wang, Z.Q.; Hemann, C.; Varnado, C.L.; Novince, Z.; Hille, R.; Goodwin, D.C.; Stuehr, D.J. "Mesohaem substitution reveals how haem electronic properties can influence the kinetic and catalytic parameters of neuronal NO synthase" Biochem. J. 2011, 433, 163.

You can view a listing of all our publications here.